Japanese Standard Car Cable,Car Cable,Heat-resistant Cable,Low-voltage Car Wire Jiangyin City Weicheng Special Cable Co.,Ltd , https://www.weichengcable.com
Japanese Standard Car Cable,Car Cable,Heat-resistant Cable,Low-voltage Car Wire Jiangyin City Weicheng Special Cable Co.,Ltd , https://www.weichengcable.com
The shipping process of the kit is too long, and the temperature during transit is excessively high. It is recommended to reduce the transportation time and ensure that the cooling system is properly maintained, especially in summer. The kit's stability appears to be poor. When removing the kit from the 2–8°C refrigerator, it is essential to allow it to equilibrate at room temperature (around 25°C) for at least 20 minutes before use. This helps all reagents reach a consistent temperature and prevents potential performance issues.
The incubator temperature should not exceed 37°C. Always check the temperature regularly and minimize the number of times you open the incubator after placing the reaction plate inside. Frequent opening can disrupt the constant temperature environment, which is particularly important for non-water-sealed incubators.
If the incubation time is insufficient, make sure to adjust the clock and set accurate timing. During the washing step, excessive impact force or prolonged soaking time may affect results. Follow the manufacturer’s instructions strictly regarding washing duration and the number of washes. Consistency is key here.
Pipetting errors may occur if the pipette does not absorb enough liquid, or if there is excess water clinging to the inner wall of the tip. Ensure that the pipette is correctly calibrated, and that the tips are properly matched and securely attached. Clean the inner walls of the tips thoroughly before use. It is best to use a new tip for each sample to avoid cross-contamination.
Another possible issue could be the quality of distilled water used. Always use fresh, high-quality distilled water to ensure optimal performance. Additionally, the substrate may not have sufficient reaction time. Be precise with timing to allow the reaction to complete fully and accurately.