PVC Cable,flexible wire,Single-core flexible wire,insulated flexible wire Jiangyin City Weicheng Special Cable Co.,Ltd , https://www.weichengcable.com
PVC Cable,flexible wire,Single-core flexible wire,insulated flexible wire Jiangyin City Weicheng Special Cable Co.,Ltd , https://www.weichengcable.com
The shipping process for the kit is taking too long, and the temperature during transit is too high. To improve this, it's recommended to reduce the transportation time, especially in summer, and ensure that cooling methods like ice packs are used effectively. The current balance of the kit is not optimal. When removing the kit from the 2–8°C refrigerator, make sure to open the lid and let it equilibrate at room temperature (around 25°C) for at least 20 minutes. This ensures all reagents reach a consistent temperature before use.
The incubator temperature should be kept below 37°C. Always check the temperature regularly and minimize the number of times the incubator is opened after placing the reaction plate inside. This helps maintain a stable environment. If using a non-water-sealed incubator, extra caution is required to prevent temperature fluctuations.
Insufficient incubation time can affect results. Make sure the timing is accurate and set the clock correctly. During the washing step, excessive impact force or prolonged soaking time may lead to errors. Follow the manufacturer’s instructions strictly regarding washing time and count the number of washes carefully.
Pipetting issues may occur if the pipette doesn’t absorb enough liquid, or if there is excess water clinging to the inner walls of the nozzle. Ensure the pipette is properly calibrated and the nozzles are clean and securely attached. It's best to use a new nozzle for each application to avoid contamination.
The quality of distilled water used might be an issue. Always use fresh, high-quality distilled water to ensure reliable results. Additionally, the substrate may not have enough reaction time. Accurate timing is crucial during this step to ensure proper development and detection.