Washing is one of the most critical steps in the ELISA test process. Although it’s not a chemical reaction itself, the washing step plays a vital role in determining the accuracy and reliability of the results. The main purpose of washing is to remove unbound enzyme-labeled substances and non-specifically adsorbed materials from the microplate wells. This ensures that only the specifically bound antigen-antibody complexes remain, which is essential for obtaining clear and accurate data. In ELISA, proteins can easily adhere to the polystyrene surface of the microplate, leading to non-specific binding. If not properly washed away, these interfering substances can cause false positives or reduce the sensitivity of the assay. Therefore, careful attention must be given to the washing procedure during the entire testing process. The specificity of the "ELISA test kit" depends on the precise interaction between the antigen's determinant and the antibody's binding site. Any deviation in the washing step can impact this delicate balance. As a result, the washing method used—whether manual or automated—must be carefully evaluated to ensure consistent and reliable outcomes. A comparison was conducted between manual washing and automatic washing methods using 753 HBsAg samples. After both washing techniques were applied, 52 samples tested positive with the manual method, while 53 showed positive results with the automatic washing machine. A t-test was performed, and the results showed no significant difference (t = 0.01, P > 0.05), indicating that both methods are equally effective in terms of detection accuracy. According to Shanghai Jinma Biological, there is no fundamental difference between manual and automated washing in ELISA tests. Both methods produce reliable results. However, the manual method is simpler, more cost-effective, and easier to implement, making it a practical choice for many laboratories. It is also highly suitable for standardization and widespread use, especially in settings where automation may not be feasible. Overall, while both washing techniques have their advantages, the manual approach remains a viable and efficient option that should not be overlooked. Proper training and standardized procedures can help ensure consistent performance, making manual washing a valuable tool in ELISA testing. The CCP1000 series high-voltage power supply module is a single-phase 220Vac input modular high-voltage power supply. The whole series adopts unified small and compact aluminum chassis with the designed output power at 1KW, and the output voltage levels at 5KV / 10KV / 20KV / 30KV / 40KV / 50KV for selection.
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The HV power module is also equipped with a DB9 interface to facilitate the bus control of embedded installation equipment. Customers can apply 0-10V signals and dry contact signals to the interface according to our interface definition to achieve comprehensive control and monitoring functions on the power supply, like high voltage start / stop, output setting and reading.
The CCP1000 high voltage power modules are equipped with complete protection functions, which can deal with sparking, short-circuit, and overload conditions. With the discharge protection circuit recommended by iDealTek-Electronics, the high-voltage module can also cope with conventional capacitor charging and discharging conditions.
At present, the high voltage power modules are mainly used in air or oil fume purification, capacitor charging and other application fields that require a high-voltage power module that can be embedded installation.