Washing is a crucial step in the ELISA test kit process. Although it's not a chemical reaction, it plays a vital role in determining the success of the experiment. The main purpose of washing in ELISA is to separate free and bound enzyme labels by removing unbound substances from the microplate. These include non-specifically adsorbed materials that may interfere with the detection process. Polystyrene plates are commonly used in ELISA, and protein adsorption is a typical issue. Therefore, it's essential to minimize non-specific binding during the reaction and remove such interfering substances through proper washing. The specificity of the ELISA test kit ensures that antigens and antibodies bind at their specific sites. This makes the washing step even more important, as any residual impurities can affect the accuracy of the results. In this study, we compared two washing methods: manual washing and automated washing. We tested 753 samples for HBsAg, and found that 52 samples were positive using the manual method, while 53 were positive with the automated one. After performing a t-test, the result showed that there was no significant difference between the two methods (t = 0.01, P > 0.05). According to Shanghai Jinma Biological, both manual and automated washing methods yield reliable results. However, the manual method is simpler, cost-effective, and efficient, making it an excellent choice for laboratories with limited resources. It’s also easier to standardize and implement on a larger scale. While automated systems offer convenience, manual washing remains a practical and valid option when performed correctly. For researchers looking for a balance between precision and affordability, manual washing is definitely worth considering. The CCP1000 series high-voltage power supply module is a single-phase 220Vac input modular high-voltage power supply. The whole series adopts unified small and compact aluminum chassis with the designed output power at 1KW, and the output voltage levels at 5KV / 10KV / 20KV / 30KV / 40KV / 50KV for selection.
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The HV power module is also equipped with a DB9 interface to facilitate the bus control of embedded installation equipment. Customers can apply 0-10V signals and dry contact signals to the interface according to our interface definition to achieve comprehensive control and monitoring functions on the power supply, like high voltage start / stop, output setting and reading.
The CCP1000 high voltage power modules are equipped with complete protection functions, which can deal with sparking, short-circuit, and overload conditions. With the discharge protection circuit recommended by iDealTek-Electronics, the high-voltage module can also cope with conventional capacitor charging and discharging conditions.
At present, the high voltage power modules are mainly used in air or oil fume purification, capacitor charging and other application fields that require a high-voltage power module that can be embedded installation.